![]() About 4% of the ingested dose of bacopaside I was detected in rat feces but none was detected in serum and urine which accorded with results from liquid chromatography tandem mass spectrometry. The method was then applied for the determination of this compound in rat serum, urine and feces after an oral dose of 15 mg/kg body weight. The percentage recoveries of bacopaside I in blood and urine were nearly 100% indicating the accuracy of the extraction. The finalised method could quantitatively determine bacopaside I in the range of 31.3-1000.0 ng and the detection and quantification limits were 1.0 and 31.3 ng, respectively. The assay was performed on polyethersulfone membrane using a polyclonal antibody raised against bacopaside I. We describe a novel immunochromatographic method for qualitative and quantitative analyses of bacopaside I, a bioactive constituent in Bacopa monnieri (L.) Wettst in biological samples. Furthermore, this method was applied for the immunohistochemical study to investigate the distribution of BI in the fresh root of Scutellaria baicalensis using immunoblotting by transferred from fresh root to the PES membrane. radix were qualitatively analyzed, and BI was visually detected by eastern blotting technique. As little as 1 mg of BI was still detected on the PES membrane under immunostaining method. Finally, a substrate was added and then BI was detected. Anti-BI MAb was bound and then antibody labeled with peroxidase directed against anti-BI MAb. BI separated by silica gel thin-layer chromatography (TLC) transferred to a polyethersulfone (PES) membrane was treated with a NaIO4 solution and reacted with bovine serum albumin (BSA) preparing BI-BSA conjugate on the PES membrane. A technique named eastern blotting was developed for the specific and easy identification of BI in the extracts of crude drugs and KMs using anti-BI monoclonal antibody (MAb). A part of its pharmaceutical properties is due to flavone glycoside, baicalin (BI). radix) is one of the most important crude drugs used in Kampo medicines (KMs).
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